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An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte). The analyte can be a drug, biochemical substance, or cell in an organism or organic sample.

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Dec 27, 2018· Assay: An assay is an analysis done to determine: The presence of a substance and the amount of that substance. Thus, an assay may be done for example to determine the level of thyroid hormones in the blood of a person suspected of being hypothyroid (or hyperthyroid). The biological or pharmacological potency of a drug.

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Assay Validation: Comprehensive experiments that evaluate and document the quantitative performance of an assay, including sensitivity, specificity, accuracy, precision, detection limit, range and limits of quantitation. Full Assay Validation will include inter-assay and inter-laboratory assessment of assay repeatability and robustness.

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The BCA Protein Assay is a popular method for colorimetric detection and quantitation of total protein. Pierce BCA Protein Assays have a unique advantage over the Coomassie dye–based assays (Bradford)–they are compatible with samples that contain up to 5% surfactants (detergents), and are affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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